mtesr plus basal medium  (InvivoGen)

Journal: STAR Protocols

Article Title: Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells

doi: 10.1016/j.xpro.2024.103592

Figure Lengend Snippet: Morphology of H1 hPSCs being sequentially differentiated into hematopoietic progenitors at three different densities In this experiment, 2.5 million cells per 10 cm dish represents an ideal initial seeding density (top row). 5 million cells per 10 cm dish is too dense, and contaminating cell-types become visible between days 2–4 (middle row). 1.5 million cells per 10 cm dish is too sparse, and cells die by day 2 (bottom row). At each stage of differentiation, cultured cells undergo morphological changes, aiding visual tracking of differentiation. Scale = 100 μm. (A) Undifferentiated hPSCs were dissociated into single cells with Accutase, seeded on Geltrex-coated 10 cm dishes in mTeSR Plus + 1 μM Thiazovivin, and allowed to recover for 24 h. At this stage, hPSC colonies are tightly-packed and appear as spiky webs. (B) Day 1 posterior primitive streak cells are larger, more rounded, and more spread out. (C) Day 2 posterior primitive streak cells are triangular or star-like in shape. Cells should spread out and achieve semi-confluency at this stage (top row). They should not be fully confluent (middle row), as over-confluency can lead to the emergence of contaminating cell-types (arrows). (D) Day 3 lateral mesoderm cells remain star-shaped and form a near-confluent monolayer. If cells are over-confluent, large swaths of contaminating mesenchyme-like cells may be visible (arrows). (E) Day 4 arterial endothelium appears more rounded and form a near-confluent monolayer. If cells are over-confluent, large swaths of contaminating mesenchyme-like cells may be visible (arrows). (F) Day 5–7 hemogenic endothelium appears as a densely packed monolayer (Day 7 hemogenic endothelium shown). (G) Between Days 8–10, semiadherent cells begin to emerge (Day 10 hematopoietic progenitors shown).

Article Snippet: mTeSR Plus basal medium (STEMCELL Technologies, 100–0276) , 1X , 400 mL.

Techniques: Cell Culture, IF-cells

Journal: STAR Protocols

Article Title: Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells

doi: 10.1016/j.xpro.2024.103592

Figure Lengend Snippet: 500 mL of mTeSR Plus + 1% penicillin/streptomycin (abbreviated “mTeSR Plus” for brevity)

Article Snippet: mTeSR Plus basal medium (STEMCELL Technologies, 100–0276) , 1X , 400 mL.

Techniques: Concentration Assay

Journal: STAR Protocols

Article Title: Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells

doi: 10.1016/j.xpro.2024.103592

Figure Lengend Snippet: 120 mL of mTeSR Plus + 1 μM Thiazovivin

Article Snippet: mTeSR Plus basal medium (STEMCELL Technologies, 100–0276) , 1X , 400 mL.

Techniques: Concentration Assay

Journal: STAR Protocols

Article Title: Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells

doi: 10.1016/j.xpro.2024.103592

Figure Lengend Snippet:

Article Snippet: mTeSR Plus basal medium (STEMCELL Technologies, 100–0276) , 1X , 400 mL.

Techniques: Recombinant, Membrane, Knock-Out, Pore Size, Sterility, Suction Filtration

Journal: iScience

Article Title: HucMSCs can alleviate abnormal vasculogenesis induced by high glucose through the MAPK signaling pathway

doi: 10.1016/j.isci.2024.111354

Figure Lengend Snippet:

Article Snippet: mTeSR TM Plus Basal Medium , Stem Cell Technologies , Cat#100-0276.

Techniques: Recombinant, Gentle, Membrane, Lysis, TUNEL Assay, Apoptosis Assay, Fluorescence, Software